Ambient Air Sampling / Air-O-Cell Sampling Procedure

Zefon's Air-O-Cell is a linear spore trap with an impaction trace and is used for the collection of airborne bio-aerosols including fungi, pollen, and particulate.

Spore traps are appropriate for air sampling at the beginning of an investigation to set a baseline and as a final check for clearance sampling.

They can also be used for monitoring during the remediation process.

A suitable pump, such as the Zefon Biopump Plus is attached to the cassette. The pump should be capable of drawing 15 litres of air per minute.

It is attached to the cassette that contains a microscope slide with a glue where the impaction trace is formed as the bioaerosol is caught.

The cassette is sent to Eurofins, where it is disassembled and the slide is stained and examined microscopically by a qualified analyst.

Sampling times depend upon the environment being sampled. The table below has suggested times for various environmental conditions.

Suggested Sampling Times
Environment Flow Rate Duration
 Outside 15 LPM  5 - 10 Minutes
 Clean Room 15 LPM 10 Minutes
 Indoor - Occupied 15 LPM 5 Minutes
 Basement or Dusty Room 15 LPM 3-5 Minutes
 Wall Cavity 15 LPM 1 Minute


• Check the expiry date of the Air-O-Cell is current and remove the seal from the bottom of the cassette and retain to reseal when sampling is complete.
• Attach the cassette to the Bio-Pump,which has been field calibrated with the Air-O-Cell rotometer and adjusted to 15 L.
• Remove the seal from the top of the cassette and retain to reseal when sampling is complete.

• Ensure that the Bio-Pump has been set for the chosen runtime from the table above. Turn on the pump and record the amount of time that air is drawn through the cassette. The Biopump will turn itself off after the time has expired and a red light will show.
• Disconnect Air-O-Cell from the Biopump and reseal cassette.
• Mark cassette with appropriate sample information and record on the Chain of Custody.
• Store cassette in a cool, dry area, away from the sun until delivered to the laboratory.

There is no maximum time for the analysis to be completed but it is advised to have the count & identification determind within the shortest possible time.

It may be easier to view the following series of Zefon Training Videos.