NextGen Sequencing

Next Generation Sequencing or NGS, involves the massively parallel sequencing of millions of DNA fragments, which minimises the need for fragment cloning used in traditional Sanger sequencing methods. The parallel sequencing provides vast amounts of data quickly and at an affordable cost. This enables in-depth analysis of entire genomes at unprecedented levels, allowing researchers to explore questions/hypotheses that previously would have taken years to answer.  


Sequencing is the process of determining the precise order of nucleotides within the genome. It includes various methods or technologies that are used to determine the order of the four nucleotides in the DNA strand. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.

 

Using NGS, we can study how you interact with the microbial co-inhabitants of your home or work – without being limited by the methodological constraints we have been subjected to in the past. NGS will allow us to determine and quantify the fullest spectrum of the microbiota in your environment.

Applications

This technology has a vast array of applications, such as:​

  • diagnosing and understanding complex diseases;
  • whole-genome sequencing;
  • analysis of epigenetic modifications;
  • mitochondrial sequencing;
  • transcriptome sequencing - understanding how altered expression of genetic variants affects an organism; and
  • exome sequencing - mutations in the exome are thought to contain up to 90% of mutations in the human genome, which leads to disease.
  • DNA techniques have been used to identify and isolate genes responsible for certain diseases, and provide the correct copy of the defective gene known as ‘gene therapy’.

Example Targets of Interest

Bacteria Panels:​

  • Actinomycetes & Nocardia
  • Mycobacteria
  • Chlamydia
  • Mycoplasma
  • Custom orders​

Mould Panels:

  • Fusarium
  • Exophalia
  • Exerohelium
  • Scedosporium
  • Sporothrix
  • Trichoderma
  • Trichosporun
  • Custom orders